Single Particle Tracking Data For "Histidine-Rich Domain Of Kinases Induce Phase Separation To Hyperphosphorylate Pol Ii Ctd"
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<strong>Experimental single-particle tracking (SPT) data supporting "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD"</strong> This dataset contains all the raw SPT data reported in "Histidine-rich domain of kinases induce phase separation to hyperphosphorylate Pol II CTD" in the form of SPT trajectories. The SPT trajectories are provided in two different formats for convenience: a CSV format and a Matlab format. Both formats are readable by Spot-On: https://spoton.berkeley.edu/ The SPT data contains "fast tracking" spaSPT data (Figure 2d) and this data was analyzed using the Matlab version of Spot-On which can be found and downloaded at: https://gitlab.com/tjian-darzacq-lab/spot-on-matlab The SPT data also contains "slow tracking" SPT data (Figure 2e). Full details about the Matlab and CSV formats are provided in the ReadMe files in the associated zip files. Please see the associated manuscript for a detailed description of how the data was acquired and analyzed. For questions about the data please contact Anders Sejr Hansen at anders.sejr.hansen {at} berkeley {dot} edu.
支持《激酶组氨酸富集结构域诱导相分离以过度磷酸化Pol II CTD》的单粒子追踪(single-particle tracking, SPT)实验数据集。本数据集包含该论文中报道的全部原始SPT数据,数据以SPT轨迹的形式存储。为便于使用,本SPT轨迹提供两种存储格式:CSV格式与Matlab格式,两种格式均可被Spot-On读取,相关工具访问地址为:https://spoton.berkeley.edu/。本SPT数据包含“快速追踪”spaSPT数据(对应论文图2d),该部分数据通过Matlab版本的Spot-On进行分析,该工具可从以下链接获取并下载:https://gitlab.com/tjian-darzacq-lab/spot-on-matlab。本SPT数据同时包含“慢速追踪”SPT数据(对应论文图2e)。关于Matlab与CSV格式的详细说明,请参见配套压缩包内的ReadMe文件。如需了解数据采集与分析的完整流程,请参阅配套发表论文。若对本数据集存在疑问,请联系Anders Sejr Hansen,电子邮箱:anders.sejr.hansen@berkeley.edu。



