Biological process enrich.

Long non-coding RNAs (lncRNAs) are among the most abundant types of non-coding RNAs in the genome and exhibit particularly high expression levels in the brain, where they play crucial roles in various neurophysiological and neuropathological processes. Although ischemic stroke is a complex multifactorial disease, the involvement of brain-derived lncRNAs in its intricate regulatory networks remains inadequately understood. In this study, we established a cerebral ischemia-reperfusion injury model using middle cerebral artery occlusion (MCAO) in male Sprague-Dawley rats. High-throughput sequencing was performed to profile the expression of cortical lncRNAs post-stroke, with subsequent validation using RT-PCR and qRT-PCR. Among the 31,183 lncRNAs detected in the rat cerebral cortex, 551 were differentially expressed between the MCAO and sham-operated groups in the ipsilateral cortex (fold change ≥2.0, P长链非编码RNA（long non-coding RNAs，lncRNAs）是基因组中丰度最高的非编码RNA类型之一，在脑组织中呈现尤为显著的高表达水平，并在多种神经生理与神经病理过程中发挥关键调控作用。尽管缺血性脑卒中是一种多因素参与的复杂疾病，但脑源性lncRNAs在其复杂调控网络中的参与机制仍未得到充分阐明。本研究以雄性斯普拉格-道利（Sprague-Dawley）大鼠为实验对象，通过大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）法构建脑缺血再灌注损伤模型。采用高通量测序技术对脑卒中后大鼠皮层lncRNAs的表达谱进行检测分析，并通过逆转录聚合酶链反应（RT-PCR）与实时定量聚合酶链反应（qRT-PCR）对测序结果进行验证。在大鼠大脑皮层检测到的31183个lncRNAs中，缺血侧皮层的MCAO组与假手术组间共有551个lncRNAs存在差异表达（倍数变化≥2.0，P