Average single-channel conductance, G, of HlyAΔ71–110 and HlyAΔ264–286 in different salt solutions.a.

aThe membranes were formed from 1% (mass/volume) asolectin dissolved in n-decane. The aqueous solutions were unbuffered and had a pH of 6. The applied voltage was 20 mV, and the temperature was 20°C. The average single-channel conductance, G (i.e. current divided by voltage), was calculated from at least 80 single events. The standard deviation of the single-channel conductance was generally below ±15%. c is the concentration of the aqueous salt solutions. The single-channel conductance of wildtype HlyA of E. coli is given for comparison [28]. The values denoted with an asterix were measured during this study with purified HlyA. n.m. means not measured.Average single-channel conductance, G, of HlyAΔ71–110 and HlyAΔ264–286 in different salt solutions.a.