Average single-channel conductance, G, of HlyAΔ71–110 and HlyAΔ264–286 in different salt solutions.a.

aThe membranes were formed from 1% (mass/volume) asolectin dissolved in n-decane. The aqueous solutions were unbuffered and had a pH of 6. The applied voltage was 20 mV, and the temperature was 20°C. The average single-channel conductance, G (i.e. current divided by voltage), was calculated from at least 80 single events. The standard deviation of the single-channel conductance was generally below ±15%. c is the concentration of the aqueous salt solutions. The single-channel conductance of wildtype HlyA of E. coli is given for comparison [28]. The values denoted with an asterix were measured during this study with purified HlyA. n.m. means not measured.Average single-channel conductance, G, of HlyAΔ71–110 and HlyAΔ264–286 in different salt solutions.a.本实验所用膜由1%（质量体积比）溶解于正癸烷（n-decane）的大豆卵磷脂（asolectin）制备而成。所用水溶液未添加缓冲体系，pH值为6。施加电压为20 mV，实验温度为20°C。单通道平均电导G（即电流与电压的比值）由至少80次单通道事件计算得出。单通道电导的标准差通常低于±15%。c代表盐水溶液的浓度。大肠杆菌野生型HlyA的单通道电导数据列于此处以供对照[28]。带有星号标记的数据为本研究中使用纯化HlyA测得的结果。n.m.表示未测量。不同盐水溶液中HlyAΔ71–110与HlyAΔ264–286的单通道平均电导G。a.