Iron(II) measurements on water column samples collected on the SIPEX II voyage of the Aurora Australis, 2012

Water samples for dissolved trace metal measurements were collected from the surface (15m) down to the 1000m using an autonomous intelligent rosette system (General Ocanics, USA) specially adapted for trace metal work and deployed on a Dyneema rope. The rosette was equipped with 12x10-L Niskin-1010X bottles specially modified for trace metal water sampling. This system has been successfully deployed on the RSV Aurora Australis during voyages au0703 and au0806. Care was taken to avoid any contamination from the ship and the operating personnel. Water samplers were processed aboard under an ISO class 5 trace-metal-clean laminar flow bench in to a trace-metal-clean laboratory container on the ship's trawl deck. All transfer tubes, filtering devices and sample containers were rinsed liberally with sample before final collection. Samples were then drawn through C-Flex tubing (Cole Parmer) and filtered in-line through 0.2 micron pore-size acid-washed capsules (Pall Supor membrane, Acropak 200).

Regular sampling depths were as follows: 1000m, 750m, 500m, 300m, 200m, 150m, 125m, 100m, 75m, 50m, 30m, 15m.

Samples were analysed within a minute of filtration. Iron(II) was detected with the luminol method combining the experimental set-up of Hansard et al. (2009) with the chemistry as described by Croot and Laan (2002). Samples were not acidified prior to analysis and were pumped directly into the flow cell without an injection valve. Care was taken to maintain a stable light field during measurements as the luminol reagent was found to be extremely sensitive to changes in light intensity.

Photons from the reaction of luminol with iron(II) were counted with a Hamamatsu photomultiplier tube housed in a light-tight box. The signal was recorded using FloZ software (GlobalFIA) and the data for each run is stored in a separate file. There is a folder for each profile that contains all the files (automatically generated by the software), which are numbered. The file numbers (e.g. sample1, sample2,...) correspond to the runs as noted in the lab book (see scans).

P.L. Croot, P. Laan (2002). Analytica Chimica Acta 466: 261-273.
S.P. Hansard et al. (2009). Deep-Sea Res. I 56: 1117-1129.

用于溶解态痕量金属测量的水样采集自表层（15米）至1000米水深，采用专为痕量金属作业优化改造的自主智能采水罗塞塔系统（autonomous intelligent rosette system，General Ocanics，美国），该系统搭载于迪尼玛（Dyneema）缆绳布放。该罗塞塔系统配备12个10升规格、经特殊改造用于痕量金属水样采集的Niskin-1010X采水瓶。
该系统已在RSV Aurora Australis（澳大利亚极光号研究船）的au0703与au0806航次中成功布放。全程采取严格防护措施，避免船舶及作业人员带来的任何污染。水样在船舶拖网甲板上的痕量金属洁净实验室舱室内，于ISO 5级痕量金属洁净层流工作台中完成样品前处理。所有传输管路、过滤装置及样品容器均在最终收集前用待采水样充分润洗。水样随后经Cole Parmer品牌C-Flex管路抽取，并通过0.2微米孔径酸洗胶囊式过滤器（Pall Supor膜，Acropak 200）完成在线过滤。
常规采样水深设置如下：1000米、750米、500米、300米、200米、150米、125米、100米、75米、50米、30米、15米。
水样在过滤完成后1分钟内完成分析。亚铁（Iron(II)）采用鲁米诺法检测，该方法整合了Hansard等（2009）的实验装置架构与Croot及Laan（2002）所描述的化学分析体系。分析前未对样品进行酸化处理，样品直接泵入流通池，未使用进样阀。鉴于鲁米诺试剂对光强变化极为敏感，测量过程中需严格维持稳定的光照环境。
鲁米诺与亚铁反应产生的光子由置于遮光箱内的Hamamatsu光电倍增管进行计数。检测信号通过FloZ软件（GlobalFIA）记录，单次运行的所有数据均存储于独立文件中。每个测深剖面对应一个专属文件夹，内含软件自动生成的所有编号文件。文件编号（如sample1、sample2等）与实验记录簿（详见扫描件）中记录的运行次序一一对应。

参考文献：
P.L. Croot, P. Laan (2002). 《Analytica Chimica Acta》466: 261-273.
S.P. Hansard等 (2009). 《Deep-Sea Research. I》56: 1117-1129.