(high-temp) No 5. Aplha diversity (16S rRNA/ITS) Output

**Output files from the No 5. Aplha diversity Workflow** page of the SWELTR
high-temp study. In this workflow we used Hill numbers to assess alpha
diversity across temperature treatments.

**alpha_wf.rdata** : contains all variables and phyloseq objects from 16s rRNA
and ITS ASV alpha diversity assessment. To see the Objects, in R run
load("alpha_wf.rdata", verbose=TRUE)

Additional files:

For convenience, we also include individual phyloseq objects (collected in zip
files) where Hill numbers have been added to the sample data tables.

_**ITS (its_alpha_objects.zip)**_ :
**its18_ps_work.rds** : phyloseq object for the FULL (unfiltered) ITS data.
**its18_ps_filt.rds** : phyloseq object for the Arbitrary filtered ITS data.
**its18_ps_perfect.rds** : phyloseq object for the PERfect ITS data.
**its18_ps_pime.rds** : phyloseq object for the PIME ITS data.

**_16S rRNA (ssu_alpha_objects.zip)_** :
**ssu18_ps_work.rds** : phyloseq object for the FULL (unfiltered) 16S rRNA
data.
**ssu18_ps_filt.rds** : phyloseq object for the Arbitrary filtered 16S rRNA
data.
**ssu18_ps_perfect.rds** : phyloseq object for the PERfect 16S rRNA data.
**ssu18_ps_pime.rds** : phyloseq object for the PIME 16S rRNA data.

Source code for the workflow can be found here:
https://github.com/sweltr/high-temp/blob/master/alpha.Rmd

SWELTR高温研究第5号α多样性工作流页面的输出文件。本工作流采用希尔数（Hill numbers）评估不同温度处理组的α多样性。

**alpha_wf.rdata**：包含16S rRNA与扩增子序列变异体（ASV）的α多样性分析中涉及的全部变量及phyloseq对象。若需查看这些对象，可在R环境中执行命令`load("alpha_wf.rdata", verbose=TRUE)`。

附加文件：
为便于使用，我们还提供了单独封装的phyloseq对象（打包为zip压缩文件），这些对象的样本数据表中已添加希尔数信息。

**ITS（its_alpha_objects.zip）**：
**its18_ps_work.rds**：对应完整（未过滤）ITS数据的phyloseq对象。
**its18_ps_filt.rds**：对应任意过滤后ITS数据的phyloseq对象。
**its18_ps_perfect.rds**：对应PERfect过滤后ITS数据的phyloseq对象。
**its18_ps_pime.rds**：对应PIME处理后ITS数据的phyloseq对象。

**16S rRNA（ssu_alpha_objects.zip）**：
**ssu18_ps_work.rds**：对应完整（未过滤）16S rRNA数据的phyloseq对象。
**ssu18_ps_filt.rds**：对应任意过滤后16S rRNA数据的phyloseq对象。
**ssu18_ps_perfect.rds**：对应PERfect过滤后16S rRNA数据的phyloseq对象。
**ssu18_ps_pime.rds**：对应PIME处理后16S rRNA数据的phyloseq对象。

本工作流的源代码可通过以下链接获取：https://github.com/sweltr/high-temp/blob/master/alpha.Rmd