Additional file 1: of Development of EMS-induced mutation population for amylose and resistant starch variation in bread wheat (Triticum aestivum) and identification of candidate genes responsible for amylose variation

Evaluation of 1035 (M3) mutant lines for amylose variation using five-time diluted standard Iodine-Potassium Iodide (I2â KI) solution. Time taken to develop blue color was recorded using a digital electronic stopwatch, while color intensity was scored as zero for no color development, + for light blue color, and ++ for deep blue color. All the data was given as a mean of three seeds. (XLSX 58 kb)采用5倍稀释的标准碘-碘化钾（Iodine-Potassium Iodide，I₂-KI）溶液，对1035个M3代突变株系的直链淀粉变异情况开展评估。通过数字电子秒表记录蓝色显色所需时长，并按规则对颜色强度评分：无显色记为0，浅蓝色记为+，深蓝色记为++。所有数据均以3粒种子的平均值呈现。（XLSX 58 KB）