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Single cell transcriptomic profiling of testicular germ cells reveals important role of phosphorylated GRTH/DDX25 in round spermatid differentiation and acrosome biogenesis during spermiogenesis

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We used single cell RNA sequencing (scRNA-seq) to analyze the gene expression dynamics of germ cell development during spermatogenesis in the testis. Testicular cells were isolated from mouse seminferous tubules using 2 step enzymatic digestion followed by filteration using 100 µm and 40 µm cell strainer and analyzed using scRNAseq.

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