Dissolved nutrients, cell counts, and nitrogen isotope measurements from Chaetoceros socialis culture experiments

This dataset is the results of 3 experiments in which the spore-forming diatom Chaetoceros socialis was grown in culture and induced into resting spores by nitrate limitation. The nitrogen isotopic composition of accumulated biomass (δ15Nbiomass; ‰ vs air) and of diatom-bound organic N (δ15Ndb; ‰ vs air) are reported at two timepoints from each experiment: prior to and after resting spore formation. Experiments 1 and 2 provide measurements for CRS mixed with vegetative Chaetoceros cells, while Experiment 3 provides measurements for isolated CRS. Fluorescence and nitrate concentration was tracked throughout each experiment, with dissolved silica, ammonium, and total reduced nitrogen also measured in Experiment 3.

本数据集为三项实验室培养实验的研究成果。实验中以硝酸盐限制诱导产孢硅藻社会角毛藻（Chaetoceros socialis）完成培养，并使其形成休眠孢子。本数据集报告了每项实验在休眠孢子形成前后两个时间点下，累积生物量的氮同位素组成（δ¹⁵N_biomass；相对于大气的千分比值）以及硅藻结合态有机氮的氮同位素组成（δ¹⁵N_db；相对于大气的千分比值）。实验1与实验2的测量对象为与营养体细胞混合的社会角毛藻休眠孢子（CRS），实验3的测量对象为分离纯化的CRS。所有实验全程追踪了荧光信号与硝酸盐浓度；实验3额外测定了溶解硅、铵态氮与总还原态氮含量。