Genome-wide maps of chromatin bound by HDAC4 in proliferating muscle cells

NGS technology was used for high-throughput profiling of DNA loci bound by HDAC4 in muscle cells in growth condition. Chromatin was immunoprecipitated from C2C12 cells and IP with anti-HDAC4 antibody. Libraries were then generated, processed with Illumina cBot for cluster generation on the flowcell, and sequenced on single-end 50 bp mode at the multiplexing level requested on HiSeq2500. ChIP-Seq standard bioinformatics analysis and functional annotation revealed that HDAC4 predominantly binds to the gene body (UTR5, introns and exons) of coding genes, rather than to upstream regulatory regions or non-coding RNAs in proliferating muscle cells. HDAC4 was IP from proliferating C2C12 cells and chromatin was sequenced