遇见数据集

Clones isolated in the yeast two-hybrid screen using the full-length BANK1.

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Figshare2015-12-02 更新2026-04-29 收录
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Clones isolated in the yeast two-hybrid screen using the full-length BANK1 (amino acids 1–785) as bait. The frame indicates if the coding sequences are in the same frame as Gal4-Activating Domain. In general polypeptides not having an in-frame (IF) position are not considered of biological interest. However, some of the proteins expressed from F1 or F2 can be translated in the correct frame, due to the existence of natural frame-shift events during translation in yeast. AA indicates the length of the clone in number of amino acids and (%) the percentage of frame corresponding to the annotation in GenBank.

本数据集包含以全长BANK1(氨基酸1~785)为诱饵,通过酵母双杂交筛选分离得到的克隆株相关信息。阅读框信息用于指示编码序列是否与Gal4激活结构域(Gal4-Activating Domain)处于同一阅读框。通常而言,不处于框内(in-frame, IF)位置的多肽不被认为具有生物学研究价值。但由于酵母翻译过程中存在天然的移码(frame-shift)事件,由F1或F2表达的部分蛋白质可按照正确阅读框进行翻译。AA代表该克隆的氨基酸残基长度,(%)则代表该克隆阅读框与GenBank注释信息匹配的比例。

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2015-12-02
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