Comparison of Soil Higher Fungal Communities between Dead and Living Abies koreana in Mt. Halla, the Republic of Korea

To improve our understanding of the relationship between soil higher fungi (belonging to Ascomycota and Basidiomycota) and Abies koreana, we surveyed A. koreana soil fungal communities in a forest in Mt. Halla, Jeju Island, Korea by next-generation sequencing (Illumina Miseq). To confirm the soil higher fungal communities, we collected two types of soils from a defined plot: soils with dead (AKDTs) and living A. koreana (AKLTs), respectively. Soil fungi were classified into 2 phyla, 19 classes, 64 orders, 133 families, 195 genera, and 229 OTUs (895,705 sequence reads). Nonmetric multidimensional scaling (NMDS) showed significantly different soil higher fungal communities between AKDTs and AKLTs (p A. koreana status (p Clavulina spp. was different between soils with AKDTs and AKLTs, suggesting that Clavulina spp. may be a crucial soil fungal species influencing A. koreana. This study will lead to a better understanding of the ecological status of A. koreana in Mt. Halla. In addition, this study could be useful for the conservation and management of A. koreana habitats.为加深对土壤高等真菌（隶属于子囊菌门（Ascomycota）与担子菌门（Basidiomycota））与朝鲜冷杉（Abies koreana）之间关联的理解，本研究采用下一代测序（next-generation sequencing）技术，依托Illumina Miseq平台，对韩国济州岛汉拿山森林内朝鲜冷杉周边的土壤真菌群落进行了调查。为明确该生境下的土壤高等真菌群落组成，我们在划定样地内采集了两类土壤样本：分别取自枯死后的朝鲜冷杉（AKDTs）周边土壤，与存活的朝鲜冷杉（AKLTs）周边土壤。本次研究共鉴定得到土壤真菌类群2门、19纲、64目、133科、195属，共计229个操作分类单元（OTUs, Operational Taxonomic Units），总序列读取数达895705条。非度量多维尺度分析（Nonmetric Multidimensional Scaling, NMDS）结果显示，AKDTs与AKLTs对应的土壤高等真菌群落结构存在显著差异（p < 0.001, R=0.878）。此外，珊瑚菌属（Clavulina spp.）的相对丰度在两类土壤样本间存在显著差异，提示珊瑚菌属（Clavulina spp.）可能是影响朝鲜冷杉生存的关键土壤真菌类群。本研究有助于进一步明晰汉拿山朝鲜冷杉的生态现状，同时可为朝鲜冷杉栖息地的保护与管理工作提供科学参考。