Raw K562 experimental data and miRNA–Hippo interaction table for the imatinib–Hippo–miRNA CML study

Raw experimental data and miRNA–Hippo interaction table for “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” Description of the data and file structure # Data for imatinib–Hippo–miRNA study in K562 cells Raw experimental data and interaction tables underlying the manuscript: Akbari S, et al. “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” (PLOS ONE, in submission). ## Files - K562_MTT_viability_raw.xlsx Raw absorbance values for 48 h MTT viability assays at multiple imatinib concentrations. - K562_AOEB_apoptosis_counts_raw.xlsx Raw AO/EB cell counts for apoptosis quantification at 48 h (control vs imatinib). - K562_ROS_DCFHDA_raw.xlsx Raw DCFH-DA fluorescence readings for ROS measurements over time. - K562_gene_qPCR_Ct_raw.xlsx Raw Ct values for Hippo pathway genes and GAPDH reference. - K562_miRNA_qPCR_Ct_raw.xlsx Raw Ct values for Hippo-related microRNAs and U6 reference. - miRNA_targets_master.csv Curated miRNA–target interaction table used for the Hippo/miRNA network. - PhaseContrast_200x_control_48h_raw.tif - PhaseContrast_200x_imatinib_48h_raw.tif - AOEB_control_raw_48h.tif - AOEB_imatinib_raw_48h.tif Raw phase-contrast and AO/EB microscopy images corresponding to morphology and apoptosis panels. ## Code All R scripts for processing GSE12211/GSE14671 and reproducing the bioinformatics analyses are available at: https://github.com/soroushakbari/cml-hippo-imatinib-k562本数据集为论文《伊马替尼处理CML K562细胞后Hippo-YAP通路与微小RNA(miRNA)的时序响应及在CD34⁺慢性髓性白血病(CML)祖细胞中的虚拟验证》的原始实验数据与miRNA-Hippo相互作用表。 数据与文件结构说明 # K562细胞伊马替尼-Hippo-miRNA研究相关数据 本数据集包含支撑该论文发表的原始实验数据与相互作用表： Akbari S 等. 《伊马替尼处理CML K562细胞后Hippo-YAP通路与微小RNA(miRNA)的时序响应及在CD34⁺慢性髓性白血病(CML)祖细胞中的虚拟验证》（已投稿至PLOS ONE） ## 文件列表 - K562_MTT_细胞活力_raw.xlsx：含不同浓度伊马替尼处理组的48小时MTT细胞活力检测原始吸光度数据 - K562_AOEB_细胞凋亡计数_raw.xlsx：48小时对照组与伊马替尼处理组的AO/EB染色细胞凋亡计数原始数据 - K562_ROS_DCFHDA_raw.xlsx：不同时间点活性氧(ROS)检测的DCFH-DA荧光强度原始读数 - K562_gene_qPCR_Ct值_raw.xlsx：Hippo信号通路基因及内参GAPDH的qPCR扩增Ct值原始数据 - K562_miRNA_qPCR_Ct值_raw.xlsx：Hippo通路相关微小RNA(miRNA)及内参U6的qPCR扩增Ct值原始数据 - miRNA_targets_master.csv：经人工整理的Hippo/miRNA调控网络所用miRNA-靶基因相互作用表 - PhaseContrast_200x_control_48h_raw.tif - PhaseContrast_200x_imatinib_48h_raw.tif - AOEB_control_raw_48h.tif - AOEB_imatinib_raw_48h.tif：对应形态学与凋亡展示板块的相差显微镜（200倍）及AO/EB染色原始显微图像 ## 代码资源 用于处理GEO数据集GSE12211、GSE14671并复现生物信息学分析的所有R脚本，可通过以下链接获取： https://github.com/soroushakbari/cml-hippo-imatinib-k562