Changes in EMT markers levels are associated with CD146 modulation.

The CD146-pCMV6-XL4 vector was transfected in MCF-7 and SKBR3 cells, CD146 expression was down-modulated (KD) in CAL51 and MDA-MB-231 cells with siRNAs. mRNAs from CD146 or siRNAs transfected cells were analyzed against mRNAs from mock transfected cells. Results are expressed as the mean of the fold change (± standard error of the mean) for at least six different mRNA preparations. Results were analyzed with the Wilcoxon matched pairs test and considered as significant when p将CD146-pCMV6-XL4载体转染至MCF-7与SKBR3细胞中；通过小干扰RNA（siRNA）使CAL51及MDA-MB-231细胞内的CD146表达发生下调（KD，敲低）。将转染CD146或小干扰RNA的细胞所提取的信使RNA（mRNA），以假转染细胞的mRNA为对照进行分析。实验结果以至少6份独立mRNA制备样本的倍数变化均值±均值标准误表示。采用威尔科克森配对符号秩检验对实验结果进行统计学分析，当p