Integrated single-cell chromatin and transcriptomic analyses of human scalp reveal etiological insights into genetic risk for hair and skin disease [scATAC-Seq]

In this study, we use single-cell ATAC sequencing (scATAC-seq) and single-cell RNA sequencing (scRNA-seq) to map chromatin accessibility and gene expression in human scalp samples from healthy patients and patients with alopecia areata We generated single-cell ATAC-seq and single-cell RNA-seq libraries from human scalp tissue from three sources: punch biopsy samples from healthy control volunteers (C_PB, n = 3), punch biopsies from patients with clinically active alopecia areata (AA, n = 5), and discarded normal peripheral surgical tissue from patients undergoing dermatologic surgeries on the scalp (C_SD, n = 7)