IGRP-specific T-cells lyse HLA-A2-positive islets in-vitro.

Data represent the percent specific cytotoxicity against the indicated targets by IGRP265–273-specific CD8 T cells. HLA-A2-restricted, tumor-antigen-specific CD8 T-cells were used as a control. 51Cr release from islets cultured in medium alone (i.e. spontaneous release) for each target was measured in 9 independent wells to calculate specific cytotoxicity as described in Materials and Methods. Data are representative of 3 independent experiments.*indicates a significant difference (pscid islets tested at the same E:T ratio; $ indicates a significant difference (pscid HHD islets between IGRP-specific CD8 T-cells and control T-cells.本数据集呈现了IGRP265–273特异性CD8 T细胞（CD8 T cells）对指定靶标的特异性细胞毒活性百分比。以人类白细胞抗原（HLA）-A2限制性肿瘤抗原特异性CD8 T细胞作为对照。按照《材料与方法》中的描述，对每个靶标仅在培养基中培养的胰岛（即自发释放组）的51铬（51Cr）释放量在9个独立孔中进行检测，以此计算特异性细胞毒活性。本数据为3次独立重复实验的代表性结果。*表示在相同效靶比（E:T ratio）下检测的pscid胰岛存在显著性差异；$表示IGRP特异性CD8 T细胞与对照T细胞之间的pscid HHD胰岛存在显著性差异。