Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking

This data set includes x, y, and z trajectories of all GABAergic synaptic vesicles that we used for the study. These GABAergic synaptic vesicles in inhibitory presynaptic terminals of living primary hippocampal neurons were labeled by single quantum dots (QDs) conjugated with anti-VGAT antibody under electrical stimulation, and were tracked three-dimensionally by using a dual-focus imaging in real-time. Each trajectory data indicates x, y, and z positions (nanometer-scale) over time from the start of imaging to the moment of vesicle fusion. The electrical stimulation to the neurons was applied for 120 s, starting from 20 s.本数据集包含本研究使用的全部γ-氨基丁酸能突触囊泡（GABAergic synaptic vesicles）的x、y、z三维轨迹数据。这些囊泡位于存活原代海马神经元的抑制性突触前末梢内，在电刺激条件下通过与抗VGAT抗体偶联的单量子点（quantum dots, QDs）完成标记，并采用实时双焦点成像技术实现三维追踪。每条轨迹数据均记录了自成像启动至囊泡融合时刻的x、y、z三维位置信息，空间尺度为纳米级。对神经元施加的电刺激时长为120秒，刺激起始时刻为成像开始后的第20秒。