Effect of menadione and plumbagin on glutathione.

The GSH and GSSG levels were expressed in nmols/mg of cell. Normal Growth refers to a non-stress condition while menadione or plumbagin stressed refers to cells stressed with 20 mM menadione or 7.0 μM plumbagin for 1 h, respectively. Menadione and Plumbagin treated and stressed refers to cells pre-treated with 0.5 mM menadione or 0.5 μM plumbagin for 1 h before being stressed with the same drugs. The means obtained for drug treated cells were compared with the normal growth condition of each strain using a t-student test and * represents statistically different results at PND–not determined.谷胱甘肽（GSH）与氧化型谷胱甘肽（GSSG）的水平以纳摩尔每毫克细胞为单位进行表述。正常生长组（Normal Growth）指非应激培养状态；甲萘醌（menadione）应激组与白花丹素（plumbagin）应激组分别指以20 mM甲萘醌、7.0 μM白花丹素处理细胞1小时构建的应激模型样本。甲萘醌与白花丹素预处理联合应激组，指先用0.5 mM甲萘醌或0.5 μM白花丹素预处理细胞1小时，再以同种药物进行应激处理的细胞样本。本研究采用t检验（t-student test），将药物处理组细胞的均值与各菌株的正常生长组均值进行统计学比较，其中*代表组间差异具有统计学意义，PND则表示未测定（not determined）。