Dual clathrin and integrin signaling systems regulate growth factor receptor activation

This collection comprises the platinum replica electron microscopy and light fluorescent data from the manuscript entitled: " Dual clathrin and integrin signaling systems regulate growth factor receptor activation. " by Alfonzo-Mendez et al. 2022. Figure 1 of this manuscript compare the structure of clathrin in HSC3 cells genome edited to express endogenous levels of EGFR-GFP after stimulation with EGF (0, 2, 5, 15, 30, 60 min time points). All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). Figure 2-4 of this manuscript performs different drug treatments or siRNA to understand the impact on clathrin structure. Treatments include EGFR, Src and b5-integrin inhibitors (Gefitinib, PP2, and cilengitide, respectively) and siEGFR, siSRC and sib5-integrin. All images are tiled montages acquired on an AMT XR111 ccd at 15000 x magnification (pixel size 1.2 nm). Figure 5 of this manuscript shows the changes in correlation between clathrin and EGFR, Src or b5-integrin after EGF stimulation. Figure 6 of this manuscript shows the changes in correlation between clathrin and different b5-integrin mutants after EGF stimulation. Figure 7 of this manuscript shows the changes in correlation between clathrin and phosphorylated EGFR or Grb2 after EGF stimulation.Software comprises MatLab codes used in the analysis of TIRF images to evaluate the correlation between clathrin and different proteins.本数据集包含Alfonzo-Mendez等人2022年发表于题为《双网格蛋白与整合素信号通路协同调控生长因子受体激活》的手稿中的铂复型电子显微镜数据与荧光显微数据。该手稿的图1对比了经基因组编辑、以内源水平表达表皮生长因子受体-绿色荧光蛋白（EGFR-GFP）的HSC3细胞，在表皮生长因子（EGF）刺激（0、2、5、15、30、60分钟时间点）后的网格蛋白结构。所有图像均为采用AMT XR111型电荷耦合器件（CCD）以15000倍放大倍率采集的拼接蒙太奇图像，像素尺寸为1.2 nm。图2至图4通过不同药物处理或小干扰RNA（siRNA）实验，探究其对网格蛋白结构的影响。所用处理手段包括表皮生长因子受体（EGFR）、Src及β5整合素抑制剂（分别为吉非替尼（Gefitinib）、PP2及西仑吉肽（cilengitide）），以及siEGFR、siSRC与sib5-integrin。所有图像均为采用AMT XR111型CCD以15000倍放大倍率采集的拼接蒙太奇图像，像素尺寸为1.2 nm。图5展示了EGF刺激后，网格蛋白与EGFR、Src或β5整合素之间的相关性变化。图6展示了EGF刺激后，网格蛋白与不同β5整合素突变体之间的相关性变化。图7展示了EGF刺激后，网格蛋白与磷酸化EGFR或生长因子受体结合蛋白2（Grb2）之间的相关性变化。本数据集还包含用于分析全内反射荧光显微镜（TIRF）图像、以评估网格蛋白与不同蛋白质间相关性的MATLAB代码。