Compounds from the kinase inhibitior library are identified as ABCB1 inhibitors by the high-throughput imaging-based efflux assay.

*Compounds that have been previously reported to interact with ABCB1. References are listed separately (Text S1).KB-V1 cells treated with the kinase inhibitor library of 193 compounds at 10 µM and 1 µM calcein AM were examined by the IncuCyteTMFLR. The object intensities of each sample were normalized to XR9576 treated cells and expressed as the percentage of inhibition. Compounds that displayed at least 50% inhibition of ABCB1-mediated efflux in all three independent experiments are listed.* 此前已有报道显示可与ABCB1（ABCB1）发生相互作用的化合物。相关参考文献已单独列出（Text S1）。 将KB-V1细胞以10 μM的193种激酶抑制剂库化合物与1 μM钙黄绿素AM（calcein AM）共同处理后，通过IncuCyte™FLR对样本进行检测。 将各样本的目标荧光强度以XR9576处理组细胞为参照进行归一化，并以抑制率百分比的形式表示。 将在全部三次独立重复实验中，均展现出至少50%ABCB1介导药物外排抑制活性的化合物列入本数据集。