A dual reporter‑gene system was developed, in which the opmCherry reporter gene driven by the constitutive PlacUV5 promoter for calibration, and the EGFP reporter gene driven by Pgap.
ARE reporter cells were exposed to 7.5 µM, 15 µM or 30 µM IRA for 24 h. The value for cells treated with vehicle DMSO (0.1% v/v) was set at 1. Results are from three separate experiments. aDue to cyto
Stop codon neighborhood DNA sequences inserted between Renilla and Firefly luciferase genes in parental dual luciferase reporter plasmid (with stop codons shown in boldface).
