Quantitative phytoplankton profiles for Pensacola Transect Station P5 collected during the R/V Bellows cruise of DeSoto Canyon, July 8, 2012

This dataset reports phytoplankton species abundance data for samples collected from the R/V Bellows during the July 8-10, 2012, cruise. Samples were taken at Pensacola Transect Station P5 (29.917 N, 87.25 W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface, and at depths of 35 and 48 meters using Niskin bottles. One liter of each sample was filtered onto a 0.45 micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.

本数据集记录了2012年7月8日至10日“贝洛斯号”（R/V Bellows）科考航次采集的浮游植物（phytoplankton）物种丰度数据。采样点位位于德索托峡谷头部的佛罗里达潘汉德尔湾（Florida Panhandle Bight）的彭萨科拉断面P5站（29.917°N，87.25°W）。本次采集了表层、35米及48米水深的1升水样，采样容器为尼斯金采水器（Niskin bottles）。每份1升水样经0.45微米硝酸纤维素滤膜过滤后风干；随后将滤膜的一部分粘贴至铝制样品台，经溅射镀膜处理后，使用JEOL JSM-6480 LV型扫描电子显微镜（scanning electron microscope）进行观测。研究对象涵盖硅藻（diatoms）、颗石藻（coccolithophores）及被甲甲藻（armored dinoflagellates），并尽可能鉴定至最低分类学阶元。在1000倍放大倍率下记录每个视野内各分类单元的细胞数，依据样品中浮游植物密度的差异，最多可检视700个视野。本数据集包含采样水深、过滤水样体积、各分类单元的计数细胞数，以及以每升细胞数计算得到的各分类单元丰度。分析过程中拍摄的显微照片亦纳入本数据集。