Effects of TCA treatment on cell cycle using FACS analysis.

*pCells were cultured in the medium without bile acids until cell density had become near 40% of confluence. Then cells were treated with or without bile acids for 24-h. The cellular DNA content was evaluated by flow cytometry. Statistical analysis was performed using Student’s t-test to compare the cell number between with and without bile acids in each cell cycle.将p细胞接种于不含胆汁酸的培养基中培养，直至细胞汇合度达到约40%。随后将细胞分别以含或不含胆汁酸的条件处理24小时。采用流式细胞术（flow cytometry）检测细胞DNA含量。采用Student t检验（Student’s t-test）进行统计学分析，对比各细胞周期中添加胆汁酸组与未添加组的细胞数目。