Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors

Explanation of included data for following publication: ------------------------------------------------------------------------------------- Functionalization of PEDOT:PSS for aptamer-based sensing of IL6 using organic electrochemical transistors DOI: 10.1038/s44328-024-00007-w Bernhard Burtscher (1), Chiara Diacci (1), Anatolii Makhinia (1,2), Marios Savvakis (1), Erik O Gabrielsson (1), Lothar Veith (3), Xianjie Liu (1), Xenofon Strakosas (1), Daniel T Simon (1) 1. Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, 60174 Norrköping, Sweden 2. RISE Research Institutes of Sweden, Digital Systems, Smart, Hardware, Printed, Bio- and Organic Electronics, 60221 Norrköping, Sweden 3. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany ------------------------------------------------------------------------------------- The data is structured according to the first figure they appear in the manuscript. Figure 2: a) Transfer curves data as .txt at various stages of the functionalization process with information of (Time / s; Gate Voltage / V; Drain Voltage / V; Drain Current / A; Gate Current / A; VR (measured voltage at the reference electrode) / V) 1) PEDOT:PSS 2) 4-ABA + PEDOT:PSS 3) Aptamer + 4-ABA + PEDOT:PSS b) Measurement data of constant sensing experiments of either IL6 (6 meas.), BSA (3 meas.) or a control without aptamers (4ABA-ETA_Control_IL6-measurement) with explanation of when analyte was changed to which concentration. c) Data of constant measurement of fully functionalized OECT in bovine plasma after incubation over night in bovine plasma and information regarding time and concentration of changes Figure 3: Raw image files of fluorescent test of AL4083 PEDOT:PSS formulation with PLL-FITC as .nd2 files: - 0.25% Gops with PLL-FITC (name: AL4083-Gops025_sample5_PLLFITC_FITC) - 0.25% Gops without PLL-FITC (name: AL4083-Gops025_sample3_4ABA_noPLLFITC_FITC_5s) - 1% Gops and 1% PSS with PLL-FITC (name: AL4083-Gops1_PSS1_sample4_PLLFITC_FITC) - 1% Gops and 1% PSS without PLL-FITC (name: AL4083-Gops1_PSS1_sample3_4ABA_noPLLFITC_FITC_5s) Figure 4: Frequency dependent OECT measurements of IL6 with PBS and different concentration of IL6. Each folder contains the nominal applied voltage (as .csv file) and the measurement voltage (in V) from the DAQ (as .tdms file in the ao1 column for each frequency). Data can then be processed with Python to fit the sine wave for each frequency to observe changes in the amplitude and phase. Figure 5: Data taken from Figure 2b and Figure 4本数据集对应下述学术论文的配套数据说明： ------------------------------------------------------------------------------------- 聚(3,4-乙烯二氧噻吩):聚苯乙烯磺酸盐（PEDOT:PSS）的功能化修饰用于基于适配体（aptamer）的白细胞介素6（Interleukin 6, IL6）有机电化学晶体管（organic electrochemical transistors, OECT）传感 DOI: 10.1038/s44328-024-00007-w 作者：Bernhard Burtscher (1), Chiara Diacci (1), Anatolii Makhinia (1,2), Marios Savvakis (1), Erik O Gabrielsson (1), Lothar Veith (3), Xianjie Liu (1), Xenofon Strakosas (1), Daniel T Simon (1) 1. 瑞典林雪平大学科学与技术系有机电子实验室，诺尔雪平 60174 2. 瑞典RISE研究院数字系统部智能硬件、印刷电子、生物电子与有机电子部门，诺尔雪平 60221 3. 德国马克斯·普朗克聚合物研究所，阿克曼韦格10号，美因茨 55128 ------------------------------------------------------------------------------------- 本数据集按照其在论文中首次出现的对应图进行分组整理。 图2： a) 不同功能化阶段的转移曲线数据，以.txt格式存储，包含字段信息：(时间/秒；栅极电压/伏特；漏极电压/伏特；漏极电流/安培；栅极电流/安培；参比电极实测电压VR/伏特)，对应功能化阶段如下： 1) 未修饰的PEDOT:PSS 2) 接枝4-氨基苯甲酸（4-Aminobenzoic Acid, 4-ABA）后的PEDOT:PSS 3) 接枝适配体与4-氨基苯甲酸后的PEDOT:PSS b) 恒定传感实验的测量数据，分别针对IL6（6次重复测量）、牛血清白蛋白（Bovine Serum Albumin, BSA，3次重复测量）以及无适配体的对照组（4ABA-ETA_Control_IL6-measurement），并标注了分析物更换为对应浓度的时间节点。 c) 完全功能化的OECT在牛血浆中过夜孵育后的恒定测量数据，包含分析物浓度变更的时间与浓度相关信息。 图3：以.nd2格式存储的AL4083型PEDOT:PSS配方与聚-L-赖氨酸-异硫氰酸荧光素（Poly-L-lysine-Fluorescein Isothiocyanate, PLL-FITC）的荧光测试原始图像文件： - 含0.25% Gops的PLL-FITC实验组（文件名：AL4083-Gops025_sample5_PLLFITC_FITC） - 含0.25% Gops的无PLL-FITC对照组（文件名：AL4083-Gops025_sample3_4ABA_noPLLFITC_FITC_5s） - 含1% Gops与1% PSS的PLL-FITC实验组（文件名：AL4083-Gops1_PSS1_sample4_PLLFITC_FITC） - 含1% Gops与1% PSS的无PLL-FITC对照组（文件名：AL4083-Gops1_PSS1_sample3_4ABA_noPLLFITC_FITC_5s） 图4：基于磷酸盐缓冲液（Phosphate Buffered Saline, PBS）与不同浓度IL6的IL6频率依赖性OECT测量数据。每个子文件夹包含标称施加电压文件（.csv格式）与数据采集系统（Data Acquisition System, DAQ）采集的测量电压文件（.tdms格式，对应各频率的ao1列数据）。后续可通过Python对各频率的正弦波进行拟合，以观察幅值与相位的变化。 图5：提取自图2b与图4的数据集