Measurement of total thiol, Glutathione reductase (GR), Catalase, Ascorbate Peroxidase and Superoxide dismutase (SOD) activity in different days’ nodule extracts of A. hypogaea.

Total thiol content was measured in 20, 40 and 80 day nodule extracts as described by Akerboom and Sies, (1981). Glutathione Reductase activity was measured spectrophotometrically at 340 nm over 2 minute by following NADPH oxidation whereas Catalase and Ascorbate Peroxidase activity was measured by following the H2O2 oxidation spectrophotometrically at 240 nm for 2 min in 20, 40, 80 day nodule extracts of A. hypogaea. SOD activity was measured in the same samples using xanthine oxidase and NBT and absorption was measured at 560 nm. Results are expressed as mean ± SD, for n = 3 experiments. P≤0.01, using one-way ANOVA.