Measurement of total thiol, Glutathione reductase (GR), Catalase, Ascorbate Peroxidase and Superoxide dismutase (SOD) activity in different days’ nodule extracts of A. hypogaea.

Total thiol content was measured in 20, 40 and 80 day nodule extracts as described by Akerboom and Sies, (1981). Glutathione Reductase activity was measured spectrophotometrically at 340 nm over 2 minute by following NADPH oxidation whereas Catalase and Ascorbate Peroxidase activity was measured by following the H2O2 oxidation spectrophotometrically at 240 nm for 2 min in 20, 40, 80 day nodule extracts of A. hypogaea. SOD activity was measured in the same samples using xanthine oxidase and NBT and absorption was measured at 560 nm. Results are expressed as mean ± SD, for n = 3 experiments. P≤0.01, using one-way ANOVA.按照Akerboom与Sies（1981）建立的实验方法，对20、40、80日龄根瘤提取物中的总巯基（total thiol）含量进行检测。谷胱甘肽还原酶（Glutathione Reductase）活性通过监测NADPH的氧化过程，采用分光光度法在340 nm波长下持续检测2分钟；过氧化氢酶（Catalase）与抗坏血酸过氧化物酶（Ascorbate Peroxidase）活性则通过监测H₂O₂的氧化过程，采用分光光度法在240 nm波长下检测2分钟，检测样本为花生（A. hypogaea）的20、40、80日龄根瘤提取物。超氧化物歧化酶（Superoxide Dismutase, SOD）活性采用黄嘌呤氧化酶联合氮蓝四唑（NBT）法在上述相同样本中检测，于560 nm波长下测定吸光度值。实验结果以平均值±标准差（mean ± SD）表示，每组开展3次独立重复实验；采用单因素方差分析（one-way ANOVA）进行显著性检验，以P≤0.01作为差异具有统计学意义的判定标准。