Transcriptomic profiling of myogenic newt A1 cells undergoing senescence induction, differentiation or serum and senescence-induced dedifferentiation

To investigate the molecular basis of senescence-induced dedifferentiation, we performed RNAseq on control proliferating cells (DMSO only) and etoposide-induced senescent cells to profile senescence induction. Additionally, we performed RNAseq on purified populations of differentiated myotubes derived from untreated A1 cells to investigate differentiation, as well as myotubes subsequently induced to dedifferentiate, either by serum exposure (10% FCS) or by exposure to 48 hour conditioned media from senescent cells (including proliferating cell conditioned media controls). We then performed gene expression profiling analysis using data obtained from RNA-seq of all 6 populations in triplicate. Comparative gene expression profiling analysis of RNA-seq data for the induction of senescence, differentiation and dedifferentiation in newt A1 cells