Study of antidiabetic activity of two novel Schiff base derived dibromo and dichloro substituted compounds in streptozotocin-nicotinamide-induced diabetic rats: pilot study

Abstract Schiff bases are aldehyde-or ketone-like chemical compounds in which an imine or azomethine group replaces the carbonyl group. Such compounds show various beneficial biological activities, such as anti-inflammation and antioxidants. The present study addresses comprehensiveevaluation of antidiabetic effect of two novel dibromides and dichlorides substituted Schiff bases substituted Schiff bases (2,2'-[1,2-cyclohexanediylbis (nitriloethylidyne)]bis[4-chlorophenol] (CNCP) and 2, 2'-[1,2-cyclohexanediylbis(nitriloethylidyne)]bis[4-bromophenol] (CNBP) with two different doses, high (LD) and low (LD) in streptozotocin and nicotinamide induced diabetic rats. The rats were separated into normal, untreated, treated and reference groups. Except for the normal group, diabetes traits were induced in the rest animals. Insulin level was measured, and the effect of the compounds on biochemical parameters of liver function and lipid profile were evaluated. High glucose and decreased insulin level are observed in the groups. The histological evaluation confirms that the hepatic architecture in the treated animals with a low dose of CNCP is quite similar to that of the normal hepatic structure and characterized by normal central vein, hepatocytes without any fatty alterations and mild red blood cell infiltration. CNCP (LD) and CNBP (HD) are more successful in enhancing cell survival in the diabetic rat’s liver and can be responsible for causing much healthier structure and notable morphology improvement.摘要 席夫碱（Schiff bases）是一类羰基被亚胺或甲亚胺基团取代的醛类或酮类类似化合物。此类化合物具备多种有益生物学活性，如抗炎、抗氧化作用。本研究针对两种新型二溴代与二氯代取代席夫碱——2,2'-[1,2-环己二基双(次氮基乙叉)]双[4-氯苯酚]（CNCP）与2,2'-[1,2-环己二基双(次氮基乙叉)]双[4-溴苯酚]（CNBP），以高剂量（HD）与低剂量（LD）两个给药梯度，全面评估其在链脲佐菌素（streptozotocin）联合烟酰胺（nicotinamide）诱导的糖尿病大鼠中的抗糖尿病效果。实验大鼠被分为正常对照组、未给药模型组、给药组与阳性对照组。除正常对照组外，其余各组大鼠均诱导建立糖尿病模型。本研究检测了各组大鼠的胰岛素水平，并评估了该类化合物对肝功能生化指标与血脂谱的影响。未给药的糖尿病模型组大鼠呈现高血糖与胰岛素水平降低的特征。组织学评估结果证实，低剂量CNCP给药组大鼠的肝脏组织结构与正常肝脏结构高度相似，表现为中央静脉形态正常、肝细胞无脂肪变性且仅伴轻度红细胞浸润。低剂量CNCP与高剂量CNBP在提升糖尿病大鼠肝细胞存活率方面效果更优，可助力构建更健康的肝脏组织结构，实现显著的形态学改善。