Relative expression analysis of genes associated with NER.

Expression analysis of NER, cell cycle regulation and DNA damage detection factors in LB1 silenced and control cells. mRNA from Sc and shLB1 U-2 OS cells was prepared at 3 days after silencing and analyzed by qRT-PCR using GAPDH as a reference gene. The change in expression of a specific gene was considered significant if the “fold change” was higher than 1.7 or lower than 0.6.本数据集针对LB1基因沉默细胞与对照细胞内的核苷酸切除修复（Nucleotide Excision Repair，NER）因子、细胞周期调控因子及DNA损伤检测因子展开表达分析。于基因沉默后第3天提取阴性对照（Sc）与shLB1转染U-2 OS细胞的mRNA，以甘油醛-3-磷酸脱氢酶（Glyceraldehyde-3-Phosphate Dehydrogenase，GAPDH）作为内参基因，通过实时定量反转录聚合酶链反应（qRT-PCR）完成表达分析。若特定基因的表达倍数（fold change）大于1.7或小于0.6，则判定其表达变化具有统计学显著性。