RNA-seq analysis of pulmonary arterial smooth muscle cells(PASMCs)stimulated with BMP9/10

Inactivation of BMP9/10 in mice leads to attenuated contractility of smooth muscle cells and decreased blood pressure.Treatment of de-differentiated PASMCs with BMP9/10 resulted in a strong increase of ACTA2 expression as measured by immunofluorescence and RT-PCR, indicating a switch from the de-differentiated, synthetic to a differentiated, contractile state. To further study the role of BMP9/10 in smooth muscle cells, we isolate PSAMCs and stimulate cells with or without BMP9/10 and perform the RNA-seq analysis. Overall design: Before the stimulation, PASMCs were cultured without any supplemet for another 48 hours. Then cells were stimulated in Promocell medium without supplements with or without BMP9 (R&D 5566-BP-010) and BMP10 (R&D 6038-BP) at 1 ng/mL and 5 ng/mL respectively.