COS7 pNLGFP INF-alpha
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
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https://data.caltech.edu/doi/10.22002/gzj7v-vps02
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资源简介:
Tilt Series Date: 2012-01-20 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting
倾斜序列采集日期:2012年1月20日
数据采集者:科拉·伍德沃德(Cora Woodward)
物种/标本:非洲绿猴(Cercopithecus aethiops,African green monkey)
细胞株:COS-7(ATCC CRL-1651)
倾斜序列采集参数:单轴倾斜模式,倾斜范围为(-60.0°, 60.0°),步长1.0°,采用恒定角度增量;电子束剂量为150.0 eV/Ų,欠焦量为-6.0 μm,放大倍数为22500倍
采集软件:UCSF tomo
上传方式:rundir
所用处理软件:imod
合作者与分工:pNLGFP原病毒构建体由加州大学洛杉矶分校(UCLA)的欧文·陈(Irvin Chen)提供;样品制备与成像工作由科拉·伍德沃德(Cora Woodward)在加州理工学院(Caltech)完成
纯化/培养条件与处理方式:将完整细胞接种于载网培养,并以脂质转染试剂(Lipofectamine)完成转染
样品制备:手动印迹法(manual blotting)
创建时间:
2024-01-31



