Characterization of replication and variations of genomic segments of a bat reovirus, BatMRV/B19-02 by RNA-seq in the infected Vero-E6 cells

In this study, we report the first isolation of a novel MRV strain from bat in South Korea. The genome sequence of BatMRV/B19-02 was determined and analyzed using phylogenetic analysis. We investigated the level abundance of viral RNA which reflected the transcription and replication of reovirus in infected cells using the next-generation sequencing RNA-seq. Furthermore, the variations/SNP was defined within all the gene segments to support the elucidative of recombination mechanisms on viruses.