EASI-FISH example data (multi-round FISH, round 4)

Example dataset that can be used for testing the EASI-FISH (Expansion-Assisted Iterative Fluorescence in situ hybridization) data analysis pipeline (https://github.com/JaneliaSciComp/multifish). Raw image data (.czi) and metadata (.mvl) are provided here. Image data was collected using a Zeiss Lightsheet Z.1 microscope. A 20× water-immersion objective (RI=1.33) was used for imaging with 1× zoom. Single-side illumination was used to reduce light exposure and imaging time. Overlap between tiles was set to 8%. Voxel size (µm): 0.23 x 0.23 x 0.42 (x, y, z). This dataset can be used with EASI-FISH example datasets (multi-round FISH, round 3 and round 5) (https://doi.org/10.25378/janelia.13622819; https://doi.org/10.25378/janelia.13622828) for registration testing. LHA3_R4_small (12GB): Two image tiles are included in a single CZI file, with 2 channels (1 FISH channel and 1 DAPI channel). Channel1: 488 (Calb2), channel 2: 405 (DAPI) and image dimension per tile: 1920 x 1920 x 413 (x, y, z).

本示例数据集可用于测试EASI-FISH（Expansion-Assisted Iterative Fluorescence in situ hybridization，扩增辅助迭代荧光原位杂交）数据分析流程（https://github.com/JaneliaSciComp/multifish）。此处提供了原始图像数据（.czi格式）与元数据（.mvl格式）。该图像数据采用蔡司Lightsheet Z.1显微镜采集，搭配20×水浸物镜（折射率RI=1.33），以1×变焦参数进行成像。实验采用单侧照明模式以降低光照暴露量并缩短成像时长，图像拼接块（tiles）间的重叠率设置为8%。体素尺寸（单位：微米）为0.23×0.23×0.42（x、y、z轴）。本数据集可与EASI-FISH示例数据集（多轮荧光原位杂交实验的第3轮与第5轮数据，https://doi.org/10.25378/janelia.13622819; https://doi.org/10.25378/janelia.13622828）配合用于配准测试。LHA3_R4_small（12GB）：单个CZI文件中包含两张图像拼接块，涵盖2个通道：1个荧光原位杂交通道与1个DAPI通道。通道1为488nm激发的Calb2标记信号，通道2为405nm激发的DAPI信号；单张拼接块的图像维度为1920×1920×413（x、y、z轴）。