Human arterial endothelial cells from pluripotent stem cells for in vitro hematopoiesis and lymphopoiesis

Arterial endothelial cells (AECs) are the founder cell for intraembryonic haematopoiesis. Here, we report a method for the efficient generation of human haemogenic DLL4+ AECs from pluripotent stem cells (PSC). Time-series single cell RNA-sequencing revealed the dynamic evolution of haematopoiesis and lymphopoiesis from endothelial stage, generating cell types with counterparts present in early human embryos. Our study describes an efficient method for producing lymphoid cells and provides insights into their endothelial and haematopoietic ontogeny, paving a new path to investigations of the human blood system. Overall design: This dataset includes a time-series single cell RNA-sequencing (scRNA-seq) analysis of arterial-hemogenic cultures at day 6, 12, 15, 19, and 25 (haematopoietic cells only on day 25), encompassing period from the generation of haemogenic endothelium to lymphoid commitment.