Establishing Guideline Values for Copper Toxicity to Marine Microbiomes

This data collection consists of water quality measurements, nutrient data and measured copper concentrations associated with an experiment exploring the effects of copper on seawater microbial communities to establish guideline values for copper toxicity to seawater microbiomes. Seawater for the experiment was collected from the AIMS jetty (19°16'38.4"S 147°03'32.1"E) in December 2020 and subsequently used in a 48-h exposure experiment. Water quality measurements (dissolved oxygen, pH and salinity) and water samples for nutrients (dissolved organic / inorganic carbon and nitrogen) were taken at 0-h and 48-h, following standard AIMS procedures. Representative water samples from the nominal experimental treatments (0, 0.1, 0.3, 1, 3, 10, 30, 100, 300 and 1000 µg L-1) were sent to Townsville Laboratory Services for dissolved copper quantification. Data was entered into excel.For full methodological and analytical details please refer to the full research publication, Thomas et al. (in press) its supplementary materials and the associated GitHub repository (GitHub - MarieCThomas/Copper.microbiome: Establishing guideline values for copper toxicity to marine microbiomes). Additionally, raw sequencing data can be found in the NCBI Sequence Read Archives under BioProject number PRJNA983933.

本数据集包含水质监测数据、营养盐数据与铜浓度实测数据，相关实验旨在探究铜对海水微生物群落的影响，进而确立铜对海水微生物组的毒性基准值。本实验所用海水于2020年12月采自澳大利亚海洋科学研究所（AIMS）码头，地理坐标为南纬19°16′38.4″、东经147°03′32.1″，随后被用于为期48小时的暴露实验。按照AIMS标准操作规程，分别在实验起始（0小时）与实验结束（48小时）两个节点采集水质监测数据（溶解氧、pH值与盐度）以及营养盐分析用海水样本，检测指标涵盖溶解有机碳、溶解无机碳及氮。从各标称实验处理组（铜浓度梯度为0、0.1、0.3、1、3、10、30、100、300及1000微克每升）中采集代表性海水样本，送至汤斯维尔实验室服务中心开展溶解铜定量检测。所有实验数据均已录入Excel表格。如需获取完整的实验方法与分析细节，请参阅Thomas等人已录用待刊的研究论文及其补充材料，以及相关GitHub仓库（GitHub - MarieCThomas/Copper.microbiome: 确立海洋微生物组铜毒性基准值）。此外，原始测序数据可在美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）的序列读取档案库（Sequence Read Archives，SRA）中获取，对应BioProject编号为PRJNA983933。