Hemagglutination inhibition assay.

Enhanced HAI titers upon booster injections and in the presence of Alum. Groups of BALB/c mice (n = 4) were immunized subcutaneously once or twice with or without the addition of the adjuvant Alum with 15, 3, 0.6 or 0.12 µg of the Qβ -gH_A1 vaccine (total amount of conjugate vaccine and not the amount of globular domain protein contained in the vaccine). HAI titers were determined 24 days after the first and second immunization.加强免疫接种及明矾佐剂（Alum）存在时血凝抑制滴度（Hemagglutination Inhibition, HAI）的升高情况。将BALB/c小鼠分为若干组，每组4只（n=4），分别以15、3、0.6或0.12微克剂量的Qβ-gH_A1结合疫苗，联合或不联合明矾佐剂（Alum）进行1次或2次皮下免疫。注：此剂量为结合疫苗的总用量，而非疫苗所含球状结构域蛋白的质量。分别于首次免疫及加强免疫后24天检测HAI。