Acute Slow Oscillation Power After Photothrombotic Stroke Wide-Field Calcium Imaging, Behavioral, and Histological Dataset

This dataset contains multimodal neuroscience data collected from 25 adult Thy1-GCaMP transgenic mice (~8–12 months of age) undergoing photothrombotic (PT) stroke, generated to assess functional and behavioral changes at acute and chronic timepoints following ischemic injury. Data were acquired from a single biological source — live mice — with tissue samples subsequently processed for post-mortem histological analysis. Three data modalities are included: widefield calcium imaging (WFCI) capturing cortex-wide neural activity via the genetically encoded calcium indicator GCaMP; behavioral data from the cylinder rearing test, which quantifies forelimb asymmetry as a measure of sensorimotor deficit; and histological data comprising both Cresyl Violet (Nissl) staining for lesion visualization and immunohistochemistry (IHC) for protein-specific labeling. Each mouse was assessed at three experimental timepoints: baseline (pre-stroke), acute (24 hours post-PT stroke), and chronic (one week post-PT stroke), with histology collected only at the post-chronic endpoint. The dataset is hierarchically organized under a top-level /data directory containing three primary modality folders: /behavior, /WFCI, and /histology. The /behavior folder is organized by mouse ID (e.g., /M01) and then by timepoint (/bsl, /acute, /weekone), containing raw video recordings, Excel-format scoring sheets, and aggregated GraphPad Prism files summarizing results across all animals. The /WFCI folder is subdivided into three processing tiers — /Raw (unprocessed imaging files), /Tier1_Derived (intermediate processed data), and /Tier2_SourceData (analysis-ready outputs organized by mouse and timepoint) — supporting reproducibility from raw acquisition through final analysis. The /histology folder contains two subfolders, /cresyl_violet and /IHC, holding stained tissue section images. File formats include TIFF images for histology, CSV and XLSX for behavioral scoring, and standard video formats for raw behavior recordings. The organizational rationale — mouse ID → modality → timepoint — enables straightforward longitudinal comparisons within subjects and cross-sectional comparisons across the cohort. Data were generated by Jake Lee and Arnav Ajay Jadav in the Landsness Lab, Department of Neurology, Washington University in St. Louis (contact: [e-mail]), and are suitable for analyses of stroke-induced changes in cortical dynamics, motor behavior, and structural pathology.本数据集包含从25只成年Thy1-GCaMP转基因小鼠（约8~12月龄）中采集的多模态神经科学数据，这些小鼠接受了光血栓卒中（photothrombotic, PT）造模，旨在评估缺血性损伤后急性与慢性时间节点的功能及行为变化。数据采集自单一生物来源——活体小鼠，后续还对组织样本进行了死后组织学分析处理。 本数据集包含三类数据模态：其一为宽场钙成像（widefield calcium imaging, WFCI），通过基因编码钙指示剂GCaMP捕获全皮层神经活动；其二为圆筒立起试验（cylinder rearing test）的行为学数据，该试验通过量化前肢不对称性以评估感觉运动缺陷；其三为组织学数据，包含用于可视化病变的焦油紫（Cresyl Violet，即Nissl）染色样本，以及用于蛋白特异性标记的免疫组织化学（immunohistochemistry, IHC）样本。 每只小鼠均在三个实验时间节点接受评估：基线（卒中前）、急性（PT卒中后24小时）与慢性（PT卒中后1周），仅在慢性终点采集组织学数据。 该数据集以层级结构组织，顶层为/data目录，包含三个核心模态文件夹：/behavior、/WFCI与/histology。其中/behavior文件夹按小鼠ID（例如/M01）分类，再按时间点（/bsl、/acute、/weekone）进一步划分，内含原始视频录像、Excel格式评分表，以及汇总所有动物实验结果的GraphPad Prism文件。/WFCI文件夹分为三个处理层级：/Raw（未处理成像文件）、/Tier1_Derived（中间处理数据）、/Tier2_SourceData（按小鼠与时间点组织的可直接用于分析的输出文件），可支持从原始采集到最终分析的全流程可复现研究。/histology文件夹包含两个子文件夹：/cresyl_violet与/IHC，用于存储染色组织切片图像。 数据集涵盖的文件格式包括：组织学数据采用TIFF图像格式，行为学评分数据采用CSV与XLSX格式，原始行为录像采用标准视频格式。 该数据集采用“小鼠ID→模态→时间点”的组织逻辑，便于开展受试者内纵向比较与队列间横断面比较。 本数据集由圣路易斯华盛顿大学神经病学系Landsness实验室的Jake Lee与Arnav Ajay Jadav生成（联系方式：[e-mail]），可用于卒中诱导的皮层动态、运动行为及结构病理变化相关分析。