Primers used for cloning of the PCLO minigene and site-directed mutagenesis.

Restriction site targets introduced to allow sequential cloning of the PCR-amplified fragments are underlined. The nucleotide replaced by site-directed mutagenesis is indicated in bold.为实现经聚合酶链式反应（Polymerase Chain Reaction，PCR）扩增所得片段的循序克隆而引入的限制性酶切位点靶点，均已用下划线标注。经定点诱变（Site-directed mutagenesis）替换得到的核苷酸，已以粗体形式标注。