Oxalate decarboxylase

In M.oryzae, the D-erythroascorbic acid (EASC) pathway is the main pathway for synthesizing oxalic acid. Mitochondria serve as intracellular power plants, with TCA cycle being the main energy metabolism process. During the process of power generation, “heat” (H2O2) is released. EASC is like a radiator, responsible for removing excess “heat” (H2O2) and ensuring normal cellular metabolism. And, the formation of oxalic acid is a product of EASC clearing excess H2O2 by TCA cycle. Meanwhile, oxalic acid forms formic acid, which enters the one-carbon cycle to form acetyl-CoA and then enters the TCA cycle. From the perspective of ATP formation, the formation of oxalic acid ensures the carbon cycle and thus the progress of energy metabolism. When the oxalic acid degradation pathway is disrupted (ΔMooxdc), the M.oryzae produces a stress response, which activates the up-regulation of oxalate oxidase and oxalate transporter protein expression to reduce the damage of excessive oxalate to the cell.