Transcriptome data: salinity adaptation in Rhithropanopeus harrisii across an estuarine gradient

Rhithropanopeus harrisii is a common estuarine crab native to the East and
Gulf Coasts of North America. Here, it is found along a broad range of
salinities, spanning from ~1 PSU to ~25 PSU along estuarine gradients. As
part of a larger study on the species' potential use of low-salinity
refuges from parasitism, we tested for differences in gene expression with
salinity using crabs from three distinct estuarine reaches along the
Pamlico River in North Carolina. We acclimated crabs collected at natural
sites with 1, 5, and 11 PSU mean salinity to 0.8, 3, and 15 PSU in the
laboratory for one week and sequenced gill tissue (second posterior gill)
from each group using mRNA-Seq. Data in this repository includes
information on sequenced samples (*csv), the de novo transcriptome
assembly (two versions: with and without expression filtering; *.fasta),
transcriptome annotation from EnTAP (two files: using the runN and runP
functions; *.tsv), a spreadsheet of normalized read count data from salmon
pseudo-alignment (*.tsv), high-quality SNPs identified from the
transcriptome sequencing with GATK (*.vcf), and three custom scripts used
in processing the SNP data (*.py and *.R). Raw sequence data is archived
in GenBank's SRA: BioProject PRJNA730785, BioSamples
SAMN19241288-SAMN19241333.