Anti-diabetic effects of fullerene C60 nanoparticle mediated by its anti-oxidant activity in the pancreas in type 1 diabetic rats

Abstract The present study aims to examine the anti-diabetic effects of fullerene C60 nanoparticle, as an anti-oxidant compound, on serum glucose level, body weight, food and water intake, and pancreatic oxidative stress in the rats with type 1 diabetes. Diabetes mellitus was induced by single intravenous injection of streptozotocine (45 mg/kg) into the tail vein of the rats. Four groups of rats were divided as follow: normal, normal treatment, diabetic, and diabetic treatment groups. Normal treatment and diabetic treatment groups received intra-orally fullerene (1 mg/ kg/daily) up to day 60 following streptozotocine injection. Oxidative stress markers in the pancreas were evaluated on day 60 after inducing diabetes mellitus. Injection of streptozotocine significantly increased serum glucose level as well as food and water intake on all experimental days; it decreased body weight on day 60. Streptozotocine increased MDA level and decreased GSH level and SOD activity in the pancreas. Fullerene significantly decreased food and water intake and increased body weight as compared with the diabetic group. Fullerene also could normalize the pancreatic MDA and GSH markers. The present study suggested that fullerene can decrease diabetic symptoms via its anti-oxidant activity in the pancreas in the rats with type 1 diabetes mellitus.摘要 本研究旨在考察作为抗氧化化合物的富勒烯C60纳米颗粒对1型糖尿病大鼠的血糖水平、体质量、摄食量、饮水量及胰腺氧化应激状态的抗糖尿病作用。本研究通过单次尾静脉注射链脲佐菌素（streptozotocine，45 mg/kg）构建大鼠糖尿病模型。将大鼠分为四组：正常对照组、正常给药组、糖尿病模型组及糖尿病给药组。正常给药组与糖尿病给药组每日经口给予富勒烯（1 mg/kg/日），直至链脲佐菌素注射后第60天。于糖尿病造模后第60天检测胰腺组织的氧化应激标志物水平。实验结果显示，链脲佐菌素注射可在所有实验天数显著升高大鼠血糖水平、摄食量与饮水量；造模后第60天时大鼠体质量下降。链脲佐菌素可升高胰腺组织中丙二醛（MDA）水平，降低谷胱甘肽（GSH）含量与超氧化物歧化酶（SOD）活性。与糖尿病模型组相比，富勒烯给药可显著降低大鼠摄食量与饮水量，并提升体质量。富勒烯还可使胰腺MDA与GSH指标恢复至正常水平。本研究表明，富勒烯可通过其在胰腺中的抗氧化活性，缓解1型糖尿病大鼠的糖尿病症状。