Additional file 2 of Efficient generation of epigenetic disease model mice by epigenome editing using the piggyBac transposon system

Additional file 2. gRNA used in this study. Primers for in vitro transcription. Primers to check for vector integration. Primers for COBRA and amplicon bisulfite sequencing. Primers for quantitative real-time RT-PCR. Primers for copy number analysis. Primers for inverse PCR (nested PCR).附加文件2：本研究使用的向导RNA（guide RNA，gRNA）。体外转录引物。载体整合验证引物。结合亚硫酸氢盐限制性分析（Combined Bisulfite Restriction Analysis，COBRA）及扩增子亚硫酸氢盐测序引物。实时定量反转录聚合酶链式反应（quantitative real-time RT-PCR）引物。拷贝数分析引物。反向聚合酶链式反应（巢式PCR，inverse PCR (nested PCR)）引物。