Data for: Antibiotic-degrading resistance changes bacterial community structure via species-specific responses

Some bacterial resistance mechanisms degrade antibiotics, potentially protecting neighbouring susceptible cells from antibiotic exposure. We do not yet understand how such effects influence bacterial communities of more than two species, which are typical in nature. Here, we used experimental multispecies communities to test the effects of clinically important pOXA-48-plasmid-encoded resistance on community-level responses to antibiotics. We found that resistance in one community member reduced antibiotic inhibition of other species, but some benefitted more than others. Further experiments with supernatants and pure-culture growth assays showed the susceptible species profiting most from detoxification were those that grew best at degraded antibiotic concentrations (greater than zero, but lower than the starting concentration). This pattern was also observed on agar surfaces, and the same species also showed relatively high survival compared to most other species during the initial hig..., ,

部分细菌耐药机制可降解抗生素，从而潜在地保护邻近的抗生素敏感细胞免受抗生素暴露的侵害。目前我们尚未明确此类效应会如何影响自然界中普遍存在的多物种细菌群落。本研究借助实验性多物种群落，探究临床相关的pOXA-48质粒编码耐药性（pOXA-48-plasmid-encoded resistance）对群落层面抗生素应答的影响。研究结果显示，单一群落成员的耐药性可降低其他物种所受的抗生素抑制作用，但不同物种的获益程度存在显著差异。后续通过上清液实验与纯培养生长实验进一步验证发现，从抗生素解毒作用中获益最多的敏感物种，正是那些在降解态抗生素浓度（大于零但低于初始浓度）下生长性能最优的物种。该模式在琼脂平板表面同样得到验证；与多数其他物种相比，上述物种在初始高[……]阶段的存活率相对更高。