Oxyrrhynchium hians (Brachytheciaceae, Bryophyta) includes several morphologically distinct and cryptic species in northwestern Europe

A study of the variable species Oxyrrhynchium hians s.l. in NW Europe based on nuclear ITS, and plastid rpl16 and trnL‑trnF, as well as morphology, revealed unsuspected species level diversity. Three taxa are distinguishable by morphology: O. distichum with complanate or sub-complanate branch leaves and long and narrow leaf lamina cells, O. hians with cordate or broadly ovate, concave leaves that are evenly arranged around the stems and branches, and O. swartzii with mostly complanate or sub-complanate branch leaves and compared with O. distichum relatively short and wide leaf lamina cells. In Sweden O. distichum grows almost exclusively on base-rich or calcareous rocks and has been recorded from a belt stretching from the Baltic Sea islands of Öland and Gotland to Dalarna and southernmost Norway, whereas the other two species grow on various substrates and have wider distributions. Oxyrrhynchium hians grows in more nutrient-rich habitats than O. swartzii and is therefore absent from re..., For the molecular portion of this study, 32 specimens of O. hians s.l. from Sweden, six from Norway, and three from Switzerland were included. One specimen each of the two closely related species O. schleicheri (R. Hedw.) Röll and O. speciosum (Brid.) Warnst., two other unambiguous members of Oxyrrhynchium (Ignatov and Huttunen 2002, Ignatov and Isoviita 2003), were included as outgroup.
Total DNA was extracted using the NucleoMag® Plant kit for DNA isolation from plant tissue (Macherey-Nagel) with the KingFisher Duo magnetic particle processor. Double stranded DNA templates were prepared by polymerase chain reaction (PCR). PCR was performed using IllustraTM Hot Start Mix RTG (GE Healthcare) in a 25 µl reaction volume according to the manufacturer’s instructions.
In all cases, the specified PCR programs were initiated by a denaturation step of 5 min at 95º C and followed by a final extension period of 10 min at 72º C. The PCR programs were, for the nuclear internal transcribed spacers 1..., , # Data from: Oxyrrhynchium hians (Brachytheciaceae, Bryophyta) includes several morphologically distinct and cryptic species in northwestern Europe

## Description of the data and file structure

Sequence alignments for ITS, rpl16, and trnL-trnF

Date of data collection: 2022-2023

Geographic range: Europe, mainly Scandinavia

Files: hians_ITS.txt (ITS); hians rpl16.txt (rpl16); hians_trnLF.txt (trnL-trnF); these are sequence alignments in FASTA format

Usage notes: The positions in the files correspond with ITS, rpl16, and trnL-trnF, as follows:

File: hians_ITS.txt

ITS positions: 1-753

ITS indel coding positions: 755-773

File: hians rpl16.txt

rpl16 positions: 1-723

rpl16 indel coding positions: 725-730

File: hians_trnLF.txt

trnL-trnF positions: 1-447

trnL-trnF indel coding positions: 449-450

## Sharing/Access information

Specimen data linked to the sequence numbers can be found in the paper (when published) at:

*

Data was derived from herbarium material locate...

本研究基于核基因组ITS序列、质体rpl16与trnL-trnF序列，并结合形态学特征，对西北欧的广义狭叶尖喙藓（Oxyrrhynchium hians s.l.）开展研究，揭示了此前未被发现的物种级多样性。通过形态特征可区分出3个分类单元：两列尖喙藓（O. distichum），其枝条叶呈扁平或近扁平状，叶肉细胞狭长；狭义狭叶尖喙藓（O. hians），叶呈心形或宽卵形、内凹，在茎枝上均匀排布；斯瓦茨尖喙藓（O. swartzii），枝条叶多呈扁平或近扁平状，与两列尖喙藓相比，其叶肉细胞相对短宽。

在瑞典，两列尖喙藓几乎仅生长于富碱或钙质岩石上，其分布记录覆盖从波罗的海岛屿厄兰岛（Öland）、哥得兰岛（Gotland）至达拉纳（Dalarna）以及挪威最南部的区域；而另外两个物种则可生长于多种基质上，分布范围更广。狭叶尖喙藓（O. hians）的生境营养水平高于斯瓦茨尖喙藓（O. swartzii），因此其无法在……（原文截断）

本研究的分子部分共纳入来自瑞典的32份广义狭叶尖喙藓（O. hians s.l.）标本、挪威的6份标本以及瑞士的3份标本。同时纳入2个近缘物种——施莱歇尔尖喙藓（O. schleicheri (R. Hedw.) Röll）和美丽尖喙藓（O. speciosum (Brid.) Warnst.）各1份标本作为外类群；这两个物种均为尖喙藓属（Oxyrrhynchium）的明确类群（Ignatov与Huttunen 2002，Ignatov与Isoviita 2003）。

总DNA提取采用NucleoMag®植物组织DNA分离试剂盒（Macherey-Nagel品牌），搭配KingFisher Duo磁珠处理器完成。双链DNA模板通过聚合酶链式反应（Polymerase Chain Reaction, PCR）制备，PCR反应使用Illustra™热启动预混液RTG（GE Healthcare品牌），反应体系为25 μL，严格按照试剂盒说明书操作。

所有PCR程序均以95℃预变性5分钟起始，最终延伸步骤为72℃保温10分钟。针对核内转录间隔区1（ITS1）的PCR程序为……

# 数据来源：狭叶尖喙藓（Oxyrrhynchium hians，隶属于青藓科Brachytheciaceae、苔藓植物门Bryophyta）在西北欧存在多个形态独特且隐蔽的物种

## 数据与文件结构
ITS、rpl16及trnL-trnF序列比对文件
数据采集时间：2022年—2023年
地理分布范围：欧洲，以斯堪的纳维亚半岛为主
文件列表：hians_ITS.txt（对应ITS序列）、hians rpl16.txt（对应rpl16序列）、hians_trnLF.txt（对应trnL-trnF序列），上述文件均为FASTA格式的序列比对文件
使用说明：文件中的位置信息与各序列对应关系如下：
文件hians_ITS.txt：ITS序列位点范围：1-753；ITS插入缺失编码位点范围：755-773
文件hians rpl16.txt：rpl16序列位点范围：1-723；rpl16插入缺失编码位点范围：725-730
文件hians_trnLF.txt：trnL-trnF序列位点范围：1-447；trnL-trnF插入缺失编码位点范围：449-450

## 共享与获取信息
与序列编号对应的标本数据可在论文发表后于以下网址获取：* *
本数据源自馆藏标本……（原文截断）