Thirty two different culture conditions tested for the expansion of human umbilical cord blood (UCB) derived hematopoietic precursor cells.
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*Plasma and serum are derived from human blood group AB. FCS: fetal calf serum.
CC1 = SCF (10 ng/ml) + IL-3 + IL-6 (100 ng/ml) + IL-1β (3 ng/ml) + EPO (1 U/ml), [25].
CC2 = Flt-3 (50 ng/ml) + MGDF (10 ng/ml), [26].
CC3 = Flt-3 (50 ng/ml) + MGDF (10 ng/ml) + IL-6 (10 ng/ml) + SCF (50 ng/ml), [27].
CC4 = Flt-3 (25 ng/ml) + SCF (25 ng/ml) + MGDF (10 ng/ml) + IL-6 (20 ng/ml), modification of [27].
▴D7 (CC4 + DMEM + 10% serum) maintained long-term expansion of UCB HSC over 7 months.
•B7 (CC2 + DMEM + 10% serum), C7 (CC3 + DMEM + 10% serum) and D4 (CC4 + RPMI +10% FCS) maintained short- term expansion for 6 weeks.
创建时间：
2010-12-30
