Thirty two different culture conditions tested for the expansion of human umbilical cord blood (UCB) derived hematopoietic precursor cells.

*Plasma and serum are derived from human blood group AB. FCS: fetal calf serum. CC1 = SCF (10 ng/ml) + IL-3 + IL-6 (100 ng/ml) + IL-1β (3 ng/ml) + EPO (1 U/ml), [25]. CC2 = Flt-3 (50 ng/ml) + MGDF (10 ng/ml), [26]. CC3 = Flt-3 (50 ng/ml) + MGDF (10 ng/ml) + IL-6 (10 ng/ml) + SCF (50 ng/ml), [27]. CC4 = Flt-3 (25 ng/ml) + SCF (25 ng/ml) + MGDF (10 ng/ml) + IL-6 (20 ng/ml), modification of [27]. ▴D7 (CC4 + DMEM + 10% serum) maintained long-term expansion of UCB HSC over 7 months. •B7 (CC2 + DMEM + 10% serum), C7 (CC3 + DMEM + 10% serum) and D4 (CC4 + RPMI +10% FCS) maintained short- term expansion for 6 weeks.