<b>Chromosome-level genome assembly of the halophytic grass </b><b><i>Zoysia macrostachya</i></b>

<i>Z. macrostachya</i> (ZN3169) was used in this study, collected by the Forest Biomaterials Research Center, National Institute of Forest Science, from Seonyu Island, Gunsan-si, Jeonbuk-do, Republic of Korea.<i>Zoysia macrostachya</i> Franch. &amp; Sav. is a halophytic perennial grass species in the Poaceae family, commonly found in the coastal regions of Korea, Japan, and East Asia. <i>Z. macrostachya</i> thrives in high-salinity environments, making it an excellent model for studying abiotic stress resilience. In this study, we present a Chromosome-level genome assembly of <i>Z. macrostachya</i>, constructed using Oxford Nanopore long reads, Illumina short reads, and Omni-C sequencing data. The assembly spans 329.78 Mb across 20 chromosomes, with a scaffold N50 of 19.24 Mb, and includes complete telomeric sequences at both ends. The assembly showed 97.8% complete BUSCOs, indicating high genome completeness. Repeat element analysis identified 44.03% of the genome as repetitive sequences, with long terminal repeats (LTRs) being the most abundant (21.09%). Gene annotation revealed 33,474 protein-coding genes, with 97.1% complete BUSCOs and 86.92% functionally characterized based on Swiss-Prot and eggNOG databases. Macrosynteny analysis highlighted highly collinear relationships with related species, providing a foundational understanding of the <i>Z. macrostachya</i> genomic structure. This high-quality genome assembly serves as a valuable resource for advancing salinity tolerance research and improving the genetic diversity of turfgrass species.<br>本研究采用的材料为大穗结缕草（*Z. macrostachya*，ZN3169），由韩国国家林业科学研究院森林生物质研究中心采集自韩国全罗北道群山市仙游岛。大穗结缕草（*Zoysia macrostachya* Franch. & Sav.）为禾本科（Poaceae）盐生多年生草本植物，广泛分布于韩国、日本及东亚沿海区域。该物种可在高盐环境中旺盛生长，是研究非生物胁迫抗性的优良模式物种。本研究结合牛津纳米孔（Oxford Nanopore）长读长测序、Illumina短读长测序及Omni-C测序数据，构建了大穗结缕草的染色体级基因组组装。该组装序列总长329.78 Mb，覆盖20条染色体，支架N50为19.24 Mb，且每条染色体两端均带有完整的端粒序列。BUSCO评估显示该组装的完整度达97.8%，表明基因组组装质量优异。重复元件分析表明，该基因组中44.03%的序列为重复序列，其中长末端重复序列（long terminal repeats，LTRs）占比最高，达21.09%。基因注释共预测得到33474个蛋白质编码基因，其BUSCO完整度为97.1%；基于Swiss-Prot与eggNOG数据库，86.92%的基因获得了功能注释。大尺度共线性分析显示，该基因组与近缘物种存在高度共线性关系，为解析大穗结缕草的基因组结构奠定了基础。该高质量基因组组装可为盐胁迫耐受性研究的推进及草坪草物种的遗传多样性改良提供宝贵的科研资源。