Table 1 in Enhancement of antroquinonol production via the overexpression of 4 - hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Table 1 Yield and regeneration rate of protoplasts from A. cinnamomea S-29.
FactorTreatmentProtoplast yieldRegeneration rate(10 6/mL)(%)Enzyme alysing enzyme45.32 ± 1.08 c46.5%snailase6.21 ± 0.10 a39.3%cellulase6.33 ± 0.12 a38.1%lysing enzyme +50.65 ± 2.07 d45.9%snailaselysing enzyme +51.81 ± 1.75 d44.6%cellulasesnailase + cellulase9.93 ± 0.13 b40.2%lysing enzyme +66.01 ± 2.46 e45.3%snailase + cellulaseOsmoticMannitol10.60 ± 0.09 B24.1%stabilizer bSorbitol10.96 ± 0.22 B32.6%Sucrose66.01 ± 2.46 C45.3%KCl7.55 ± 0.17 A17.1%MgSO4127.24 ± 3.56 D54.6%Age c (days)236.7 ± 2.08 a11.4%388.97 ± 2.70 b36.9%4127.24 ± 3.56 c54.6%539.11 ± 1.82 a54.4%MgSO d (M) 40.675.85 ± 2.02 A41.8%0.8127.24 ± 3.56 B54.6%1.0196.87 ± 3.56 D55.7%1.2187.52 ± 3.56 C50.4%
a Other parameters: 4-day-old germlings digested for 4 h in 0.8 M sucrose with 20 mg/mL enzyme.
b Other parameters: 4-day-old germlings digested for 4 h in 0.8 M osmotic stabilizer with a mixture of 20 mg/mL enzymes.
c Other parameters: the germlings digested for 4 h in 0.8 M MgSO with a 4 mixture of 20 mg/mL enzymes.
d Other parameters: 4-day-old germlings digested for 4 h in MgSO with a 4 mixture of 20 mg/mL enzymes. The bars labeled with different letters (a-e or A- D) indicate protoplast yield are significantly different (p <0.05).