File S1 - Rice Chalky Ring Formation Caused by Temporal Reduction in Starch Biosynthesis during Osmotic Adjustment under Foehn-Induced Dry Wind

Table S1, P values, ratio to control, and relative expression of 39 differentially expressed genes analyzed in the developing caryopsis after 24 h dry wind treatment. Table S2, Primer sequences used in quantitative RT-PCR. Figure S1, (A) Nano-ESI mass spectrum (positive ion mode) of the ethanol-insoluble fraction from 13C-pre-fixed kernels after partial starch-degradation treatment (see Materials and Methods). (B) Expanded mass spectrum of the range m/z = 203–205 in (A). Since sodiated glucose (m/z = 203) was the most abundant ion among those containing glucose ([Glc + H]+, [Glc + Na]+, and [Glc + K]+) produced by ESI in the Exactive Orbitrap MS, the signal intensity of ions with m/z = 204 (C513CH12O6+23Na; Mm = 204.0561693) and m/z = 203 (C6H12O6+23Na; Mm = 203.0527693) was used to calculate the isotopic ratio for glucose. (C) Nano-ESI mass spectrum (positive ion mode) of the buffer solution used in the experiment (blank spectrum).
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