Figure 3D-E primary data

Figure 3D-E legend. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”). Where indicated, the cells were also treated with 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”) and/or a cocktail of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) at 1:2000 dilution. IFN-λ1 mRNA levels were measured by RT-qPCR and normalized to 18S rRNA. In D, IFN-λ1 mRNA levels are plotted relative to the lytic+casp-i+anti-IFN Abs sample. The lytic+casp-i+anti-IFN Abs samples in E were sorted based on tdTomato expression and the fold enrichment in IFN-λ1 mRNA levels compared to unsorted (“bulk”) samples are plotted.图3D-E的图例。将BC3-IFN-βp-tdTomato报告细胞以20 ng/ml的TPA处理48小时，以诱导裂解周期（"lytic (+TPA)"）。如实验标注所示，部分细胞同时用10 μM的泛半胱天冬酶抑制剂IDN-6556（"casp-i"）和/或1:2000稀释的抗I型干扰素及其受体的抗体组合（"anti-IFN Abs"）进行处理。采用RT-qPCR检测IFN-λ1 mRNA水平，并以18S rRNA作为内参进行标准化。在图D中，IFN-λ1 mRNA水平以lytic+casp-i+anti-IFN Abs样品为参照进行绘图。在图E中，lytic+casp-i+anti-IFN Abs样品依据tdTomato表达进行分选，并绘制与未分选（"bulk"）样品相比IFN-λ1 mRNA的富集倍数变化。